Hydrogen peroxide catalase coursework

These projects are not just designed to test your knowledge of the particular topic but also to test how well you understand the scientific method that is how the experiments demonstrate Hydrogen peroxide catalase coursework you are hypothesising and also the practical techniques important in lab work.

This results in a bigger proportion of molecules having a kinetic energy greater than that of the activation energy.

Hydrogen peroxide is naturally produced by the body and broken down by catalase. Conversely, dietary fish oil increased catalase activity in the heart, and kidneys of mice.

Free Science essays

Push the plunger on the syringe and immediately start the stopclock. This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules.

Whilst I'm sure your experiment won't contain such a gaping hole in it's method it will surely contain some weaknesses that you will need to show in your results and account for in your next section.

Any heavy metal ion such as copper cations in copper II sulfate can act as a noncompetitive inhibitor of catalase. Health and safety checked, September Downloads Investigating an enzyme-controlled reaction: Cells make the enzyme catalase to remove hydrogen peroxide.

Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Just pumping the heat up might speed the decomposition time but this does not prove that the enzyme is more effective at that temperature as it may just be the heat that is causing the decomposition.

This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0. This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.

The reaction is exothermic. This means the amount of substrate could have been different for each repeat, even though I used the same concentration.

Effect of Substrate Concentration on the Rate of Activity of Catalase

Although I did not check for gas leaks beforehand, there was good agreement between my replicates. Considering the shape of curve is significant for analysis. How will this affect the results. This easy test, which can be seen with the naked eye, without the aid of instruments, is possible because catalase has a very high specific activitywhich produces a detectable response, as well as the fact that one of the products is a gas.

To prevent this I had to dry out the barrel and syringe before commencing the procedure. Discs of potato react too slowly.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. This would result in a greater probability of successful collisions, and therefore more enzyme-substrate complexes being produced. As I have mentioned earlier, this decreases in steps of 3.

Bevor Sie fortfahren...

Cells make the enzyme catalase to remove hydrogen peroxide. I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.

Hydrogen peroxide

Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas. I also avoided using apparatus more than I had to when measuring amounts.

Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide.

Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by. Apr 30,  · I had to change the volume of hydrogen peroxide used from 5cm 3 to 4cm 3 because the first reaction with % hydrogen peroxide went too fast to collect oxygen at a measurable rate.

When I repeated the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of michaelferrisjr.coms: 5.

Hydrogen peroxide is the toxic by-product of respiration, and would cause cell damage if it were not quickly removed. Cells produce the enzyme catalase which breaks hydrogen peroxide down into two harmless substances - water and oxygen.

Jul 05,  · Ask a Scientist - Reaction of the enzyme catalase with hydrogen peroxide How does the activity of Catalase vary with temperature? Hemoprotein Examples What other proteins have the heme group in them?

part of the michaelferrisjr.com Archives Course on chemical kinetics. Excellent material. Hydrogen peroxide is released due to many metabolic reactions in the body, which gives toxicity to the living cells.

An Oasis of Healing

Hence, there is a chemical need to remove hydrogen peroxide in a very rapid rate. Hence, there is a chemical need to remove hydrogen peroxide in a very rapid rate. AP Biology Lab #2 Enzyme Catalysis Overview: In this lab you will observe the conversion of hydrogen peroxide (H2O2) to water and oxygen gas by the enzyme catalase, and then measure the amount of oxygen generated.

You will also calculate the rate of an enzyme-catalyzed reaction.

Hydrogen peroxide catalase coursework
Rated 3/5 based on 1 review
Yahoo ist jetzt Teil von Oath